Below is a list of PCR primers for insects. Upon use, please reconfirm the sequence by checking the original papers.
Drosophila melanogaster
kl-5 (Y-linked)
kl5-1: GCTATAAACTTTAACGCAGTC
kl5-2: GCAAGCAATATGCTCTC
note: annealing: 50C, Anbutsu & Fukatsu (2003) AEM; can be used for sex diagnosis
AttA
AttA241F: CCGGAAACACTCAAAGTGGTC (Tm=65.6)
AttA381R: TGAATAAATTGGCATGGGCC (Tm=66.4)
annealing: 55C, Anbutsu & Fukatsu (2003) AEM
ef1-alpha
elf_23F: TTAACATTGTGGTCATTGGCCA
elf_123R: CTTCTCAATCGTACGCTTGTCG
note: 100-bp product (for QPCR)Drosophila hydei
CO2 (mitochondrial)
CO2-489f: CATGAACAATTCCCGCTTTAGG
CO2-590r: ATTGTCCATAAAATAATCCTGGGC
note: 100-bp product (for QPCR)Bombyx mori
EF2（elongation factor-2）
EF2-f1: AAGAAGCGGAATATCCGCAA
EF2-r1: GTTGGAGCTCAAGAAGAGCA
note: 475 bp
doublesex
Bmdsx-2: AACCATGCCACCACTGATACCAAC（Tm=53.6）
Bmdsx-rev1: GCACAACGAATACTGCTGCAATCG（Tm=53.6）
Bmdsx-E2F1: CCGTCCCCTCGGAGACGCTTGTGGAG（Tm=63.6）
Bmdsx-MR1: CTGCTGCAATCGCCATTGATGC（Tm=52.9）
note: Bmdsx-2 + Bmdsx-rev1 -> Bmdsx-E2F1 + Bmdsx-MR1 (female: 428bp, male: 179bp)Lepidoptera
Tpi
Tpi-F: GGTCACTCTGAAAGGAGAACCACTTT
Tpi-R: CACAACATTTGCCCAGTTGTTGCCAA
note: Jiggins et al., 2001
ND5 (mitochondrial)
V1-HR: CCTGTTTCTCTGCTTTAGTTTAGTTCA
SPR2-TR: CCATAAGTARATAAAYTWGGYATAAATCA
COI (mitochondrial)
COI-321F: GATTTTTTGGACATCCTGAAG
COI-689R: CTAAAATTACTCCTGTTAATCC
note: Originally designed for Eurema but can be used widely in Lepidoptera (Narita et al., 2007 FEMS Microbiol. Ecol.).
